A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Increase NP yield |
Metabolic engineering > Modular pathway engineering |
In module 1, we employed a modified GAL system to overexpress the genes of the naringenin biosynthesis pathway and investigated their synergistic action. In module 2, we simultaneously up-regulated acetyl-CoA production and down-regulated fatty acid biosynthesis in order to increase the precursor supply, malonyl-CoA. In module 3, we engineered the tyrosine biosynthetic pathway to eliminate the feedback inhibition of tyrosine and also down-regulated competing pathways. |
NPC490716 |
Anthocyanins |
n.a. |
90 mg/L |
20-fold |
PMID[28707470] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Increase NP yield |
n.a. |
an efficient 2,3- BDO producing yeast strain was rapidly constructed with up to 25-copy genome integration of 2,3-BDO biosynthesis pathway. Further strain engineering was conducted by multiplex disruption of ADH1, PDC1, PDC5 and MTH1 using a 2μ-based replicative CRISPR Cas9 plasmid containing donor DNAs. |
NPC490725 |
argyrin J |
n.a. |
50.5g/L |
3.9-fold |
PMID[32006629] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Increase NP yield |
n.a. |
Then the Lightning GTR-CRISPR was applied to simplify yeast lipid metabolism through two-round ORF deletions for these eight genes. After the first-round deletion of FAA1, FAA4, POX1, and ARE2, the plasmid was anti-selected by growing the cells on 5-FOA medium, and the second-round deletions of PAH1, LPP1, DPP1, and ARE1 were carried out. |
NPC35656 |
artemisinic acid |
19.93 mg/L |
559.52 mg/L |
30-fold |
PMID[30837474] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Increase NP yield |
n.a. |
n.a. |
NPC490733 |
bacillothiazol 1 |
n.a. |
8.7+/-0.85mg/l |
40-fold increase |
PMID[18485776] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Increase NP yield |
Metabolic engineering |
combination of a metabolic engineering strategy with fermentation technology optimization can be used to obtain significant amounts of the high-value sesquiterpene α-santalene |
NPC490741 |
bacillothiazol 4 |
n.a. |
0.0052 Cmmol (Cmmol glucose)(-1); final 0.036 Cmmol (g biomass)(-1) h(-1) |
4-fold improvement over the reference strain |
PMID[22938570] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Increase NP yield |
n.a. |
n.a. |
NPC490790 |
chitosanase |
n.a. |
252 μg/L |
critical intermediates by 32-fold; additional 45-fold through culture optimization |
PMID[25981946] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Increase NP yield |
n.a. |
n.a. |
NPC314309 |
cinnamycin |
n.a. |
548 μg/L |
critical intermediates by 32-fold; additional 45-fold through culture optimization |
PMID[25981946] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Increase NP yield |
n.a. |
n.a. |
NPC490791 |
cis-N-methylstylopine |
n.a. |
676 μg/L |
critical intermediates by 32-fold; additional 45-fold through culture optimization |
PMID[25981946] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Increase NP yield |
n.a. |
n.a. |
NPC118429 |
Citrulline |
n.a. |
80 μg/L |
critical intermediates by 32-fold; additional 45-fold through culture optimization |
PMID[25981946] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Heterologous biosynthesis; Increase NP yield |
Transporter engineering; and regulatory factor modulating. |
n.a. |
NPC313821 |
lincomycin |
n.a. |
0.5 g/L |
n.a. |
PMID[25769286] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Heterologous biosynthesis; Increase NP yield |
n.a. |
Yeast chromosomes integration; Cell factories establishment. |
NPC313821 |
lincomycin |
n.a. |
0.17 g/L |
n.a. |
PMID[25769286] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
NP de novo biosynthesis |
n.a. |
n.a. |
NPC490856 |
long-chain Dicarboxylic acids |
n.a. |
0.85 mg/L |
n.a. |
PMID[29771352] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Heterologous biosynthesis; Increase NP yield |
n.a. |
UGTPgs characterization; Structural modeling; Protein chimeric. |
NPC313179 |
lutein |
n.a. |
1.4 mg/L |
n.a. |
PMID[24603359] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
NP de novo biosynthesis |
n.a. |
n.a. |
NPC99088 |
lycopene |
n.a. |
1.55 mg/L |
n.a. |
PMID[29771352] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
NP de novo biosynthesis |
n.a. |
n.a. |
NPC99088 |
lycopene |
n.a. |
1.86 mg/L |
n.a. |
PMID[29771352] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Increase NP yield |
n.a. |
n.a. |
NPC490867 |
medium chain fatty acid(C6-C10, MCFAs) |
n.a. |
108 mg/L |
n.a. |
PMID[29386648] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Increase NP yield |
n.a. |
n.a. |
NPC79943 |
Naringenin |
n.a. |
14.54 mg/L |
n.a. |
PMID[28634125] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
NP de novo biosynthesis |
n.a. |
n.a. |
NPC490923 |
N-Methylanthranilate Production |
n.a. |
152 mg/L |
n.a. |
PMID[29771352] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Increase NP yield |
n.a. |
Firstly, we synthesized or cloned the genes encoding dammarenediol-II synthase (DS), PgUGT74AE2 and UGTPg1 from P. ginseng. Furthermore, the anti-colon cancer activities of both 3β-O-Glc-DM and 20S-O-Glc-DM were demonstrated by in vitro and in vivo assays. we engineered S. cerevisiae by introducing the codon-optimized genes encoding DS together with PgUGT74AE2 or UGTPg1. The engineered strains were optimized to increase the production of DM glucosides by integrating multi-copy DS and UGT genes into yeast via the CRISPR/Cas9 system, overexpressing several key enzymes of the upstream biosynthetic pathway, down-regulating the competitive branch pathway and overexpressing the transcriptional activator HAC1. |
NPC93081 |
Ornithine |
n.a. |
2.4 g/L |
n.a. |
DOI[10.1039/C8GC04066D] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Increase NP yield |
n.a. |
n.a. |
NPC93081 |
Ornithine |
n.a. |
2.3 mg/L |
n.a. |
PMID[28634125] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Increase NP yield |
n.a. |
n.a. |
NPC93081 |
Ornithine |
n.a. |
185 mg/L |
n.a. |
PMID[29386648] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Increase NP yield |
n.a. |
n.a. |
NPC490932 |
oxidized derivative medicagenic acid |
n.a. |
20.38 mg/L |
n.a. |
PMID[28634125] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Increase NP yield |
n.a. |
n.a. |
NPC11433 |
Proline |
n.a. |
26.57 mg/L |
n.a. |
PMID[28634125] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Increase NP yield |
n.a. |
n.a. |
NPC161571 |
resveratrol |
n.a. |
33.1+/-5.6mg/l |
n.a. |
PMID[18485776] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Green production |
n.a. |
overexpressing seven heterologous enzymes and five native enzymes and the strain YC1053 overexpressing seven heterologous enzymes and four native enzymes |
NPC490983 |
rhizomide A |
n.a. |
336.8 mg/L |
n.a. |
PMID[32004707] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Heterologous biosynthesis; Increase NP yield |
n.a. |
UGTPgs characterization; Structural modeling; Protein chimeric. |
NPC331737 |
sesquiterpenes |
n.a. |
42 mg/L |
n.a. |
PMID[26032089] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
NP de novo biosynthesis |
n.a. |
n.a. |
NPC490999 |
spinosad |
n.a. |
5.31 mg/L |
n.a. |
PMID[28634125] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
NP biosynthesis |
n.a. |
We next introduced 11 heterologous genes and modified 12 native yeast genes to construct a yeast strain capable of producing 8-prenylkaempferol with high efficiency; relocating GmOMT2 into mitochondria (higher pH than cytoplasm) of the 8-prenylkaempferol–producing yeast strain |
NPC123965 |
Squalene |
n.a. |
7.2 mg/L |
n.a. |
DOI[10.1016/j.scib.2021.03.002] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Increase NP yield |
n.a. |
Firstly, we synthesized or cloned the genes encoding dammarenediol-II synthase (DS), PgUGT74AE2 and UGTPg1 from P. ginseng. Furthermore, the anti-colon cancer activities of both 3β-O-Glc-DM and 20S-O-Glc-DM were demonstrated by in vitro and in vivo assays. we engineered S. cerevisiae by introducing the codon-optimized genes encoding DS together with PgUGT74AE2 or UGTPg1. The engineered strains were optimized to increase the production of DM glucosides by integrating multi-copy DS and UGT genes into yeast via the CRISPR/Cas9 system, overexpressing several key enzymes of the upstream biosynthetic pathway, down-regulating the competitive branch pathway and overexpressing the transcriptional activator HAC1. |
NPC123965 |
Squalene |
n.a. |
5.6 g/L |
n.a. |
DOI[10.1039/C8GC04066D] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Increase NP yield |
n.a. |
n.a. |
NPC123965 |
Squalene |
n.a. |
648.63 mg/L |
n.a. |
PMID[31690080] |
A new engineered strain of Saccharomyces cerevisiae |
Saccharomyces cerevisiae |
n.a. |
Heterologous biosynthesis; Increase NP yield |
n.a. |
Yeast chromosomes integration; Cell factories establishment. |
NPC233950 |
thiolactomycin |
n.a. |
92 mg/L |
n.a. |
PMID[26032089] |