Organism Name: A new engineered strain of Escherichia coli [DAR1, GPP2, dhaB1, dhaB2, dhaB3, dhaBX, yqhD, glpK, gldA, gap, gaIP, glk]| Organism ID: | NPO41847 |
| Organism Name: | A new engineered strain of Escherichia coli [DAR1, GPP2, dhaB1, dhaB2, dhaB3, dhaBX, yqhD, glpK, gldA, gap, gaIP, glk] |
| Native / Engineered Organism: | Engineered Organism |
| Matched Taxonomy Level: | Strain |
| Organism External ID: | NCBI_Taxonomy_ID[ n.a.] |
| Organism Synonyms: | |
| Genus: | Escherichia |
| Family: | Enterobacteriaceae |
| Kingdom: | n.a. |
| SuperKingdom: | Bacteria |
Individual Natural Products| Natural Product ID | Prefer Name (or InChiKey) | IUPAC Name | Number of Targets | Number of Activity Records |
|---|---|---|---|---|
| NPC51700 | Ursolic Acid | (1S,2R,4aS,6aR,6aS,6bR,8aR,10S,12aR,14bS)-10-hydroxy-1,2,6a,6b,9,9,12a-heptamethyl-2,3,4,5,6,6a,7,8,8a,10,11,12,13,14b-tetradecahydro-1H-picene-4a-carboxylic acid | 175 | 680 |
NPs produced by Co-culture Organisms (for Co-culture organisms, the full organism list can be found HERE)| Organism Name | Producer Organism | Inducer Organism | NP ID | NP Name | Effect of Co-culture | Reference |
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NPs produced by Engineered Organisms (for Engineered organisms, the full organism list can be found HERE)| Organism Name | Parent Organism Name | Engineered Gene in Organism | Purpose of Engineering | Engineering Category | Engineering Description | NP ID | NP Name | NP Yeild of Parent Organism | NP Yeild of Engineered Organism | Yeild Change | Reference |
|---|---|---|---|---|---|---|---|---|---|---|---|
| A new engineered strain of Escherichia coli | Escherichia coli | DAR1, GPP2, dhaB1, dhaB2, dhaB3, dhaBX, yqhD, glpK, gldA, gap, gaIP, glk | Increase NP yield | n.a. | A metabolic pathway was designed that diverted from dihydroxyacetone phosphate (DHAP), a central metabolite in the glycolytic pathway (Figure 8), by introducing genes encoding glycerol-3-phosphate dehydrogenase (DAR1), glycerol-3-posphate-phosphatase (GPP2), both from S. cerevisiae, glycerol dehydratase (dhaB1, dhaB2, dhaB3), and the reactivation enzyme for glycerol dehydratase (dhaBX) from Klebsiella pneumoniae, and finally the native E. coli oxidoreductase (yqhD). In addition, carbon flux to competing pathways was reduced by deleting the genes for glycerol kinase (glpK) and glycerol dehydrogenase (gldA) as well as downregulation of glycerol aldehyde dehydrogenase activity (gap). Furthermore, the phosphoenolpyruvate transferase system (PTS) for glucose uptake was replaced by a galactose permease (galP) to prevent the loss of phosphorenol pyruvate for glucose uptake and an ATP-dependent phosphorylation of glucose by glucokinase (glk). | NPC51700 | ursolic acid | n.a. | titer: 135g/L; yield: 0.51g 1,3-propanediol/g glucose | n.a. |
PMID[14580573] |
The Quantitative Compositions of NPs in Organism| Organism ID | NP ID | Organism Material Preparation | Organism Part | NP Quantity (Standard) | NP Quantity (Minimum) | NP Quantity (Maximum) | Quantity Unit | Reference |
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